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Thursday 18 April 2013
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Host preference of fungal communities in a tropical forest: evidences obtained with 454 pyrosequencing of fungal ITS


Heidy Schimann1, Eliane Louisanna1, Claude Murat2 and Marc Buée2

1- INRA Joint Research Unit ECOFOG, campus agronomique, BP 709, 97387 Kourou cedex, French Guiana
2- INRA Joint Research Unit IAM, Champenoux, 54280 Champenoux, France

In tropical forests, Fungi contribute to the recycling of organic matter, the nutrition of plants and can also modulate the composition of plant communities. It is now quite clear that the number of fungal species in tropical and subtropical climate is higher than in temperate ones. Several studies have already highlighted some of the environmental and biotic factors that could modulate and structure the composition of fungal communities of tropical forests. In this study, we made the hypotheses that tree specie modulates the structure of fungal communities in soil and litter. In particular, a- and b-diversities of fungal communities in soils and litters are partly dependent of the specie of the tree above. We selected two experimental sites to address this question: a long-term plantation of tropical trees and a closed plot of natural forest. After extraction of total DNA from soil and litter samples, the fungal ITS region of DNA has been amplified and sequenced by 454 pyrosequencing. After filtering, 300 000 sequences constituted the dataset and were used to calculate rarefaction curves and various diversity indices, and to determine the effect of tree species on the composition of fungal communities both in plantation and in natural forest. Results showed a significant spatial heterogeneity and nonrandom associations with the different tree species but with few variations of richness or evenness of fungal species in the plantation. In natural forest, the tree specie above modulated in a less proportion the structure of fungal communities. Taken together, these results suggest that host preference could partly explained the structuration of fungal communities inhabiting soils and litters in tropical forests.

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    31 July 2015, by BRANDNER Melissa

    The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park , Poland. A variety of scientists attended from all over the globe to learn, share and inspire with unique stories of metabarcoding.
    The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year’s workshop. And our hats go off to them for the organizational skills, warmth and hospitality. During the week, experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding, sparking conversations between the attendees. The end of the first day saw flash talks from all participants of the event, creating an icebreaker and showing the wide variety of applications for metabarcoding, including, dietary studies, environmental health, fundamental ecology and exploration of rare and ancient habitats.
    Pierre Taberlet (who is rumoured to run 8000 PCR a day!) taught technical aspects such as DNA extraction in (...)

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