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Thursday 18 April 2013
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Gastrointestinal microbiota of wild and inbred individuals in two House Mouse subspecies assessed using high throughput parallel pyrosequencing


Jakub Kreisinger 1,2, Dagmar Čížková 2, Jaroslav Piálek 2

1 Department of Zoology, Faculty of Science, Charles University in Prague, Czech Republic
2 Academy of Science of the Czech Republic, Institute of Vertebrate Biology, Brno, Czech Republic

The effect of gastrointestinal tract microbiota (GTM) on the physiological and health status of the host has obtained considerable interest in past years. Various captive bread animal species are used in the experimental branch of this research. It is, however, poorly known to that extent does the GTM of captive and/or inbred individuals resemble the natural composition and variation of GTM in wild populations. We performed 16S rDNA barcoding of gut microbiota in 30 house mouse (Mus musculus) individuals from wild population and from wild-derived inbred strains using 454 pyrosequencing. Sequenced individuals were selected according to balanced 2x2 experimental design: their origin into wild (14) vs. inbred (16) and M. m. musculus (15) vs. M. m. domesticus (15). We compared alpha diversity (i.e. number of operational taxonomic units), beta diversity (i.e. interindividual variability) and microbiota composition across the four groups. We found no difference between musculus and domesticus subspecies, indicating that gut microbiota does not play role in the speciation process. Both inbred and wild population showed the same level of microbial diversity. We found strong differentiation of wild and inbred population based on the microbiota composition. Laboratory mouse represents the most abundant model to study the effect of gut microbiota on host metabolism, immunity and neurology. We suggested, that the existence of distinct microbiota in laboratory-kept mice, which differs in composition from the natural wild microbiota should be further considered in the biomedical research.

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  • BRANDNER Melissa
    • University of Nordland
    • Bodø (Norway)
  • BROCHMANN Christian
    • National Centre for Biosystematics
    • Oslo (Norway)
  • CHARITON Anthony
    • CSIRO Land and Water
    • Lucas Heights NA (Australia)
  • DEAGLE Bruce
    • University of Victoria
    • Victoria (Canada)
  • Eric Coissac
    • LECA
    • Grenoble (France)
  • KASAPIDIS Panagiotis
    • Hellenic Center for Marine Research
    • Irakleion, Crete (Greece)
    • Université de Genève
    • Genève 4 (Switzerland)
  • TABERLET Pierre
    • LECA,
    • Grenoble (France)
    • Centre for GeoGenetics
    • Copenhagen (Denmark)
  • ZINGER Lucie
    • IBENS
    • Paris (France)
  • Vodka, Bison and Metabarcoding

    31 July 2015, by BRANDNER Melissa

    The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park , Poland. A variety of scientists attended from all over the globe to learn, share and inspire with unique stories of metabarcoding.
    The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year’s workshop. And our hats go off to them for the organizational skills, warmth and hospitality. During the week, experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding, sparking conversations between the attendees. The end of the first day saw flash talks from all participants of the event, creating an icebreaker and showing the wide variety of applications for metabarcoding, including, dietary studies, environmental health, fundamental ecology and exploration of rare and ancient habitats.
    Pierre Taberlet (who is rumoured to run 8000 PCR a day!) taught technical aspects such as DNA extraction in (...)

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