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Thursday 18 April 2013
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“Next generation biodiversity”: Combination of molecular and morphological approaches to estimate biodiversity in lagoonal sediments

Cristina PAVLOUDI

Benthic macrofaunal diversity has been traditionally estimated though means of classical taxonomy, i.e. handsorting and taxonomic identification of the macrofaunal organisms according to the relevant literature. This approach, although leading to undoubtedly results especially when the taxonomic identification is conducted by an expert in the field, is time consuming and laborious. The alternative approach is based on the use of DNA metabarcoding, combined with next-generation sequencing; this approach promises to minimize the time and effort needed to complete such a project since it allows the automated identification of multiple species from the sediment samples. This project involves the combination of both the aforementioned techniques in the estimation of benthic biodiversity of five different lagoons. The area under study is Amvrakikos Gulf (Ionian Sea), which is considered as one of the most ecologically important wetland systems in Greece. Based on previous studies in this area, there is extensive knowledge on the sediment’s macrofaunal diversity. Also, the genetic diversity of specific species has been estimated using molecular markers such as the cytochrome oxidase subunit I (COI) gene. This previous knowledge will be the basis of this metabarcoding project, which will employ next generation sequencing for the assessment of the lagoonal biodiversity patterns.

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Editorial board
  • BRANDNER Melissa
    • University of Nordland
    • Bodø (Norway)
  • BROCHMANN Christian
    • National Centre for Biosystematics
    • Oslo (Norway)
  • CHARITON Anthony
    • CSIRO Land and Water
    • Lucas Heights NA (Australia)
  • DEAGLE Bruce
    • University of Victoria
    • Victoria (Canada)
  • Eric Coissac
    • LECA
    • Grenoble (France)
  • KASAPIDIS Panagiotis
    • Hellenic Center for Marine Research
    • Irakleion, Crete (Greece)
  • PAWLOWSKI Jan
    • Université de Genève
    • Genève 4 (Switzerland)
  • TABERLET Pierre
    • LECA,
    • Grenoble (France)
  • WILLERSLEV Eske
    • Centre for GeoGenetics
    • Copenhagen (Denmark)
  • ZINGER Lucie
    • IBENS
    • Paris (France)
Editorial
  • Vodka, Bison and Metabarcoding

    31 July 2015, by BRANDNER Melissa

    The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park , Poland. A variety of scientists attended from all over the globe to learn, share and inspire with unique stories of metabarcoding.
    The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year’s workshop. And our hats go off to them for the organizational skills, warmth and hospitality. During the week, experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding, sparking conversations between the attendees. The end of the first day saw flash talks from all participants of the event, creating an icebreaker and showing the wide variety of applications for metabarcoding, including, dietary studies, environmental health, fundamental ecology and exploration of rare and ancient habitats.
    Pierre Taberlet (who is rumoured to run 8000 PCR a day!) taught technical aspects such as DNA extraction in (...)

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