Home > Events > 1 Metabarcoding spring school in French Alps (5-9 March 2012) > Studying microbial diversity

Thursday 15 March 2012

Studying microbial diversity

ZINGER Lucie

Characterizing microbial diversity has become crucial to understand ecosystem functioning and sustainability. The advent of Sanger sequencing and now next-generation sequencing technologies overcame the methodological challenges posed by working with microbes in nature, forming large, highly diverse, but elusive populations. However, this methodological revolution must consider conceptual and methodological matters to correctly interpret microbial diversity and its patterns. During this talk, I will present advances, gaps and caveats of these recent approaches for studying microbial diversity. I’ll discuss potentials and limitations of the available methodologies, from sampling to sequence analysis, and suggest alternative ways to incorporate results in a conceptual and methodological framework.

Twitter : @Metabarcoding
Editorial board
  • BRANDNER Melissa
    • University of Nordland
    • Bodø (Norway)
  • BROCHMANN Christian
    • National Centre for Biosystematics
    • Oslo (Norway)
  • CHARITON Anthony
    • CSIRO Land and Water
    • Lucas Heights NA (Australia)
  • DEAGLE Bruce
    • University of Victoria
    • Victoria (Canada)
  • Eric Coissac
    • LECA
    • Grenoble (France)
  • KASAPIDIS Panagiotis
    • Hellenic Center for Marine Research
    • Irakleion, Crete (Greece)
  • PAWLOWSKI Jan
    • Université de Genève
    • Genève 4 (Switzerland)
  • TABERLET Pierre
    • LECA,
    • Grenoble (France)
  • WILLERSLEV Eske
    • Centre for GeoGenetics
    • Copenhagen (Denmark)
Editorial
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    31 July 2015, by BRANDNER Melissa

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    The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year’s workshop. And our hats go off to them for the organizational skills, warmth and hospitality. During the week, experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding, sparking conversations between the attendees. The end of the first day saw flash talks from all participants of the event, creating an icebreaker and showing the wide variety of applications for metabarcoding, including, dietary studies, environmental health, fundamental ecology and exploration of rare and ancient habitats.
    Pierre Taberlet (who is rumoured to run 8000 PCR a day!) taught technical aspects such as DNA extraction in (...)

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