Water bodies are a rich source of suspended environmental DNA (eDNA) and recent work has demonstrated that this source includes DNA shed by macrofauna in addition to that of living microbes. This aquatic ’macrobial’ eDNA can provide evidence that a given organism is present and may also provide information about population or biomass density. As one part of my PhD research (University of Notre Dame, USA) on genetic monitoring of aquatic macrofauna using eDNA I am investigating the relationship between fish abundance and eDNA abundance. In this presentation I will discuss some findings and some methods for optimizing this relatively new approach to genetic monitoring. I will additionally discuss some new aquatic eDNA work just beginning in our research group that will utilize a metagenetic/metabarcoding approach with next-generation sequencing.
Home > Events > 1 Metabarcoding spring school in French Alps (5-9 March 2012) > Presentations > Investigating fish abundance through quantification of aqueous (...)
Investigating fish abundance through quantification of aqueous environmental DNA
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BRANDNER Melissa
- University of Nordland
- Bodø (Norway)
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BROCHMANN Christian
- National Centre for Biosystematics
- Oslo (Norway)
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CHARITON Anthony
- CSIRO Land and Water
- Lucas Heights NA (Australia)
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DEAGLE Bruce
- University of Victoria
- Victoria (Canada)
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Eric Coissac
- LECA
- Grenoble (France)
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KASAPIDIS Panagiotis
- Hellenic Center for Marine Research
- Irakleion, Crete (Greece)
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PAWLOWSKI Jan
- Université de Genève
- Genève 4 (Switzerland)
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TABERLET Pierre
- LECA,
- Grenoble (France)
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WILLERSLEV Eske
- Centre for GeoGenetics
- Copenhagen (Denmark)
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ZINGER Lucie
- IBENS
- Paris (France)
Vodka, Bison and Metabarcoding
The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park , Poland. A variety of scientists attended from all over the globe to learn, share and inspire with unique stories of metabarcoding.
The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year’s workshop. And our hats go off to them for the organizational skills, warmth and hospitality. During the week, experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding, sparking conversations between the attendees. The end of the first day saw flash talks from all participants of the event, creating an icebreaker and showing the wide variety of applications for metabarcoding, including, dietary studies, environmental health, fundamental ecology and exploration of rare and ancient habitats.
Pierre Taberlet (who is rumoured to run 8000 PCR a day!) taught technical aspects such as DNA extraction in (...)
Also in this section
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- From Swaziland to the UK - next generation sequencing on bat faeces
- Enhancing freshwater ecosystem biomonitoring: Defining and testing a next generation sequencing biodiversity identification framework.
- Trophic transfer, bioaccumulation and effects of contaminants in terrestrial food webs
- Construction of curated taxonomic reference libraries for DNA metabarcoding
- Taphonomy of Vertebrate Dirt DNA
- Habitat use and food preferences of a large herbivore: the moose, Alces alces in Poland revealed by radio-tracking and molecular diet analysis
- Inventorying tropical rich ecosystems with DNA barcoding
- Investigating fish abundance through quantification of aqueous environmental DNA
- Designing new metabarcodes
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