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Friday 30 September 2011

Metabarcoding spring school in French Alps (5-9 March 2012)

Eric Coissac, TABERLET Pierre

In the context of the european project EcoChange, the Laboratoire d’Ecologie Alpine (LECA) organizes a spring school about DNA Metabarcoding applied to biodiversity assessment and diet analysis.

Many ecological questions rely on the knowledge of the list of species involved in the studied process. Tackling this demand using standard methods (botanical relevés, micro-histology, palynology) is often a difficult task that relies on highly qualified persons. DNA barcoding has proposed to use short standardized genomic sequences (barcode) as a character in taxonomical identification. DNA Metabarcoding use the same principle that associate DNA sequences to taxa for estimating biodiversity of an environmental sample. As all living organism spread cells in their environment, a PCR amplicon obtained with adequate primers from the total DNA extracted from an environmental sample can be consider as a mirror of the biodiversity present in the environment. The pertinent choice of the primer pair allows to focus on a specific group as, for example, plants. Then the sequencing of a large number of individual DNA molecules of the PCR amplicon using next generation sequencer allows to establish a list of taxa present in the sample. Depending of the sample (e.g. soil, water, permafrost, feces), DNA metabarcoding can be applied to many ecological studies such as plant community analysis, past ecosystem reconstruction, or diet assessment.

We propose a spring school including lectures and practical sessions on metabarcoding. This school is open for twenty participants (student or researcher)

Main lecturers

  • Christian Brochmann
  • Eric Coissac
  • François Pompanon
  • PIerre Taberlet
  • Eske Willerslev
  • Lucie Zinger

Previsional program

Monday 5Tuesday 6 Wednesday 7Thursday 8Friday 9
9h - 10h Welcome Eric Coissac
Designing new metabarcodes
François Pompanon
Diet analysis
Pierre Taberlet
Biodiversity estimation from soil analysis
Lecture
10h - 11h Pierre Taberlet
Introduction to DNA metabarcoding
Christian Brockmann
Construction of curated taxonomic reference libraries
Lucie Zinger
Bacterial and Fungal biodiversity estimation
Eric Coissac
From sequences to biodiversity
Lecture
11h00 - 11h30 Coffee break Coffee break Coffee break Coffee break Coffee break
11h30 - 12h 30 Four participant
presentations
Four participant
presentations
Four participant
presentations
Four participant
presentations
Four participant
presentations
12h30 - 14h30 Lunch Lunch Lunch Lunch Lunch
14h30 - 17h30 Practical session :
Group A : Soil sampling
Group B : Marker design
Practical session :
Group A : Marker design
Group B : Soil sampling
Practical session :
Group A : DNA extraction
Group B : Data analysis
Practical session :
Group A : Data analysis
Group B : DNA extraction
Skiing afternoon

Application

The number of participants will be limited to 20. Applications including a short CV and a short motivation letter should be sent to spring2012 at metabarcoding.org, with subject: “ECOCHANGE DNA Metabarcoding School 2012” no later than DECEMBER 15th 2011.

Accomodation

All lectures and practicals will be in a small village (Pinsot) near Grenoble. You will stay in an hotel : Le pic de la belle étoile with all facilities (restaurant, swimming pool...). Lectures will take place in the same hotel.

Workshop Costs

Workshop by itself is free. Full board accommodations will be paid by participants directly to the hotel (approx. 800 € for six nights + all meals, Sunday the 4th to Saturday the 10th).

Twitter : @Metabarcoding
Editorial board
  • BRANDNER Melissa
    • University of Nordland
    • Bodø (Norway)
  • BROCHMANN Christian
    • National Centre for Biosystematics
    • Oslo (Norway)
  • CHARITON Anthony
    • CSIRO Land and Water
    • Lucas Heights NA (Australia)
  • DEAGLE Bruce
    • University of Victoria
    • Victoria (Canada)
  • Eric Coissac
    • LECA
    • Grenoble (France)
  • KASAPIDIS Panagiotis
    • Hellenic Center for Marine Research
    • Irakleion, Crete (Greece)
  • PAWLOWSKI Jan
    • Université de Genève
    • Genève 4 (Switzerland)
  • TABERLET Pierre
    • LECA,
    • Grenoble (France)
  • WILLERSLEV Eske
    • Centre for GeoGenetics
    • Copenhagen (Denmark)
Editorial
  • Vodka, Bison and Metabarcoding

    31 July 2015, by BRANDNER Melissa

    The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park , Poland. A variety of scientists attended from all over the globe to learn, share and inspire with unique stories of metabarcoding.
    The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year’s workshop. And our hats go off to them for the organizational skills, warmth and hospitality. During the week, experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding, sparking conversations between the attendees. The end of the first day saw flash talks from all participants of the event, creating an icebreaker and showing the wide variety of applications for metabarcoding, including, dietary studies, environmental health, fundamental ecology and exploration of rare and ancient habitats.
    Pierre Taberlet (who is rumoured to run 8000 PCR a day!) taught technical aspects such as DNA extraction in (...)

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