Home > Events > 1 Metabarcoding spring school in French Alps (5-9 March 2012) > Presentations > Designing new metabarcodes

Friday 2 March 2012

Designing new metabarcodes

Eric Coissac

Environmental DNA metabarcoding is at the same time similar and different with the classical taxonomical DNA Barcoding. The aims of environmental studies and the technical constraints oblige most of the time to compare several sets of primers and eventually to design new primers dedicated to a specific study. I will discuss during this presentation about bioinformatics approaches allowing an objective estimation of the relative qualities of several PCR primer pairs. Based on these method, it is possible to develop software selecting new primer pairs by maximizing these criteria in the context of a set of experimental constraints defined by the biologist.

Twitter : @Metabarcoding
Editorial board
  • BRANDNER Melissa
    • University of Nordland
    • Bodø (Norway)
  • BROCHMANN Christian
    • National Centre for Biosystematics
    • Oslo (Norway)
  • CHARITON Anthony
    • CSIRO Land and Water
    • Lucas Heights NA (Australia)
  • DEAGLE Bruce
    • University of Victoria
    • Victoria (Canada)
  • Eric Coissac
    • LECA
    • Grenoble (France)
  • KASAPIDIS Panagiotis
    • Hellenic Center for Marine Research
    • Irakleion, Crete (Greece)
  • PAWLOWSKI Jan
    • Université de Genève
    • Genève 4 (Switzerland)
  • TABERLET Pierre
    • LECA,
    • Grenoble (France)
  • WILLERSLEV Eske
    • Centre for GeoGenetics
    • Copenhagen (Denmark)
Editorial
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    The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year’s workshop. And our hats go off to them for the organizational skills, warmth and hospitality. During the week, experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding, sparking conversations between the attendees. The end of the first day saw flash talks from all participants of the event, creating an icebreaker and showing the wide variety of applications for metabarcoding, including, dietary studies, environmental health, fundamental ecology and exploration of rare and ancient habitats.
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